Cell biology of Enteroviruses and Type 1 Diabetes (T1D)
T1D is caused by a complex interplay of genetic predisposition and environmental factors, which may include viruses. Recently a rapid increase in incidence of T1D could be observed worldwide, which is too fast to be caused by genetic factors alone and indicates an increasing role of environmental factors. It has been reported that infections with Human Enteroviruses (HEVs) such as Coxsackievirus B5 (CVB5) can trigger or precipitate the onset of T1D. However, it is not know with certainty if and how HEVs are involved in the development of T1D.
Previous studies in the lab have shown that infection of insulin producing cells with CVB5 reduces the levels of mature insulin secretory granule (SG) proteins and insulin release. The aim of my project is to investigate the fate of mature SG proteins upon CVB5 infection and to identify and quantify peptides presented on MHC class I molecules by mass spectrometry.